Fig 1: Claudin-2 expression decreases in CPE from slc4a10 ko mice. (A) Immunohistochemical detection of claudin-2 in slc4a10 wt and ko CPE, as indicated. Bar graph on the right show the semi-quantitation of the immunofluorescence in slc4a10 wt and ko mouse CPE (wt: n = 5, ko: n = 3). (B) High magnification micrographs of claudin-2 immunoreactivity in slc4a10 wt and ko CPE, as indicated. Arrows indicate the luminal membrane, while arrowheads indicate the basolateral membrane. *indicates statistical significance. Cell nuclei were visualized by Topro nuclear staining (blue).